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mouse reg3b antibody  (Bio-Techne corporation)


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    Bio-Techne corporation mouse reg3b antibody
    Mouse Reg3b Antibody, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 94/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 94 stars, based on 20 article reviews
    mouse reg3b antibody - by Bioz Stars, 2026-03
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    A) Fluorescence micrographs of T-cell factor 4 (Tcf4, green) and proliferating cell nuclear antigen (PCNA, red) protein localization in the small intestine of wild-type Tcf7l2 wt/wt /VillinCreER T2 ( Tcf7l2 wt/wt ) and knockout Tcf7l2 flox/flox /VillinCreER T2 ( Tcf7l2 flox/flox ) mice, with antibiotics (ATB) and without antibiotic treatment, over time after tamoxifen administration. The images show disruptions in the epithelial architecture of Tcf4-deficient mice that are partially restored by antibiotic treatment. The insets show increased magnification of the framed areas. Scale bar: 50 μm. B) Histological sections show the mislocalization and expression changes of the Paneth cell markers lysozyme (Lyz) and regenerating islet-derived 3 beta <t>(Reg3b)</t> seven days after tamoxifen administration. Yellow arrowheads indicate Lyz-positive cells on the villi; the white arrowheads in the lower left image show that the crypt bases in Tcf4 + epithelium are not positive for Reg3b, whereas they produce Reg3b in Tcf4-deficient epithelium (white arrowheads in the lower right figure). Scale bar: 50 μm. C) Kaplan-Meier survival curves for Tcf7l2 knockout mice treated with either antibiotics or water after tamoxifen induction. Statistical significance (p = 0.034) shows that antibiotic treatment increases survival. D) Analysis of the composition of the microbiome in the distal small intestine of Tcf7l2 wt/wt and Tcf7l2 flox/flox mice. The pie chart shows the bacterial distribution in Tcf4-proficient mice (a solid black point on the outer perimeter of the chart) and Tcf4-deficient mice (an empty black ring). The principal component analysis (PCA) below the diagram illustrates the variance between bacterial populations, indicating shifts in the microbiota associated with the Tcf7l2 status. Tcf7l2, transcription factor 7 like 2.
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    A) Fluorescence micrographs of T-cell factor 4 (Tcf4, green) and proliferating cell nuclear antigen (PCNA, red) protein localization in the small intestine of wild-type Tcf7l2 wt/wt /VillinCreER T2 ( Tcf7l2 wt/wt ) and knockout Tcf7l2 flox/flox /VillinCreER T2 ( Tcf7l2 flox/flox ) mice, with antibiotics (ATB) and without antibiotic treatment, over time after tamoxifen administration. The images show disruptions in the epithelial architecture of Tcf4-deficient mice that are partially restored by antibiotic treatment. The insets show increased magnification of the framed areas. Scale bar: 50 μm. B) Histological sections show the mislocalization and expression changes of the Paneth cell markers lysozyme (Lyz) and regenerating islet-derived 3 beta <t>(Reg3b)</t> seven days after tamoxifen administration. Yellow arrowheads indicate Lyz-positive cells on the villi; the white arrowheads in the lower left image show that the crypt bases in Tcf4 + epithelium are not positive for Reg3b, whereas they produce Reg3b in Tcf4-deficient epithelium (white arrowheads in the lower right figure). Scale bar: 50 μm. C) Kaplan-Meier survival curves for Tcf7l2 knockout mice treated with either antibiotics or water after tamoxifen induction. Statistical significance (p = 0.034) shows that antibiotic treatment increases survival. D) Analysis of the composition of the microbiome in the distal small intestine of Tcf7l2 wt/wt and Tcf7l2 flox/flox mice. The pie chart shows the bacterial distribution in Tcf4-proficient mice (a solid black point on the outer perimeter of the chart) and Tcf4-deficient mice (an empty black ring). The principal component analysis (PCA) below the diagram illustrates the variance between bacterial populations, indicating shifts in the microbiota associated with the Tcf7l2 status. Tcf7l2, transcription factor 7 like 2.
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    A) Fluorescence micrographs of T-cell factor 4 (Tcf4, green) and proliferating cell nuclear antigen (PCNA, red) protein localization in the small intestine of wild-type Tcf7l2 wt/wt /VillinCreER T2 ( Tcf7l2 wt/wt ) and knockout Tcf7l2 flox/flox /VillinCreER T2 ( Tcf7l2 flox/flox ) mice, with antibiotics (ATB) and without antibiotic treatment, over time after tamoxifen administration. The images show disruptions in the epithelial architecture of Tcf4-deficient mice that are partially restored by antibiotic treatment. The insets show increased magnification of the framed areas. Scale bar: 50 μm. B) Histological sections show the mislocalization and expression changes of the Paneth cell markers lysozyme (Lyz) and regenerating islet-derived 3 beta <t>(Reg3b)</t> seven days after tamoxifen administration. Yellow arrowheads indicate Lyz-positive cells on the villi; the white arrowheads in the lower left image show that the crypt bases in Tcf4 + epithelium are not positive for Reg3b, whereas they produce Reg3b in Tcf4-deficient epithelium (white arrowheads in the lower right figure). Scale bar: 50 μm. C) Kaplan-Meier survival curves for Tcf7l2 knockout mice treated with either antibiotics or water after tamoxifen induction. Statistical significance (p = 0.034) shows that antibiotic treatment increases survival. D) Analysis of the composition of the microbiome in the distal small intestine of Tcf7l2 wt/wt and Tcf7l2 flox/flox mice. The pie chart shows the bacterial distribution in Tcf4-proficient mice (a solid black point on the outer perimeter of the chart) and Tcf4-deficient mice (an empty black ring). The principal component analysis (PCA) below the diagram illustrates the variance between bacterial populations, indicating shifts in the microbiota associated with the Tcf7l2 status. Tcf7l2, transcription factor 7 like 2.
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    A) Fluorescence micrographs of T-cell factor 4 (Tcf4, green) and proliferating cell nuclear antigen (PCNA, red) protein localization in the small intestine of wild-type Tcf7l2 wt/wt /VillinCreER T2 ( Tcf7l2 wt/wt ) and knockout Tcf7l2 flox/flox /VillinCreER T2 ( Tcf7l2 flox/flox ) mice, with antibiotics (ATB) and without antibiotic treatment, over time after tamoxifen administration. The images show disruptions in the epithelial architecture of Tcf4-deficient mice that are partially restored by antibiotic treatment. The insets show increased magnification of the framed areas. Scale bar: 50 μm. B) Histological sections show the mislocalization and expression changes of the Paneth cell markers lysozyme (Lyz) and regenerating islet-derived 3 beta <t>(Reg3b)</t> seven days after tamoxifen administration. Yellow arrowheads indicate Lyz-positive cells on the villi; the white arrowheads in the lower left image show that the crypt bases in Tcf4 + epithelium are not positive for Reg3b, whereas they produce Reg3b in Tcf4-deficient epithelium (white arrowheads in the lower right figure). Scale bar: 50 μm. C) Kaplan-Meier survival curves for Tcf7l2 knockout mice treated with either antibiotics or water after tamoxifen induction. Statistical significance (p = 0.034) shows that antibiotic treatment increases survival. D) Analysis of the composition of the microbiome in the distal small intestine of Tcf7l2 wt/wt and Tcf7l2 flox/flox mice. The pie chart shows the bacterial distribution in Tcf4-proficient mice (a solid black point on the outer perimeter of the chart) and Tcf4-deficient mice (an empty black ring). The principal component analysis (PCA) below the diagram illustrates the variance between bacterial populations, indicating shifts in the microbiota associated with the Tcf7l2 status. Tcf7l2, transcription factor 7 like 2.
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    A) Fluorescence micrographs of T-cell factor 4 (Tcf4, green) and proliferating cell nuclear antigen (PCNA, red) protein localization in the small intestine of wild-type Tcf7l2 wt/wt /VillinCreER T2 ( Tcf7l2 wt/wt ) and knockout Tcf7l2 flox/flox /VillinCreER T2 ( Tcf7l2 flox/flox ) mice, with antibiotics (ATB) and without antibiotic treatment, over time after tamoxifen administration. The images show disruptions in the epithelial architecture of Tcf4-deficient mice that are partially restored by antibiotic treatment. The insets show increased magnification of the framed areas. Scale bar: 50 μm. B) Histological sections show the mislocalization and expression changes of the Paneth cell markers lysozyme (Lyz) and regenerating islet-derived 3 beta <t>(Reg3b)</t> seven days after tamoxifen administration. Yellow arrowheads indicate Lyz-positive cells on the villi; the white arrowheads in the lower left image show that the crypt bases in Tcf4 + epithelium are not positive for Reg3b, whereas they produce Reg3b in Tcf4-deficient epithelium (white arrowheads in the lower right figure). Scale bar: 50 μm. C) Kaplan-Meier survival curves for Tcf7l2 knockout mice treated with either antibiotics or water after tamoxifen induction. Statistical significance (p = 0.034) shows that antibiotic treatment increases survival. D) Analysis of the composition of the microbiome in the distal small intestine of Tcf7l2 wt/wt and Tcf7l2 flox/flox mice. The pie chart shows the bacterial distribution in Tcf4-proficient mice (a solid black point on the outer perimeter of the chart) and Tcf4-deficient mice (an empty black ring). The principal component analysis (PCA) below the diagram illustrates the variance between bacterial populations, indicating shifts in the microbiota associated with the Tcf7l2 status. Tcf7l2, transcription factor 7 like 2.
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    Image Search Results


    A) Fluorescence micrographs of T-cell factor 4 (Tcf4, green) and proliferating cell nuclear antigen (PCNA, red) protein localization in the small intestine of wild-type Tcf7l2 wt/wt /VillinCreER T2 ( Tcf7l2 wt/wt ) and knockout Tcf7l2 flox/flox /VillinCreER T2 ( Tcf7l2 flox/flox ) mice, with antibiotics (ATB) and without antibiotic treatment, over time after tamoxifen administration. The images show disruptions in the epithelial architecture of Tcf4-deficient mice that are partially restored by antibiotic treatment. The insets show increased magnification of the framed areas. Scale bar: 50 μm. B) Histological sections show the mislocalization and expression changes of the Paneth cell markers lysozyme (Lyz) and regenerating islet-derived 3 beta (Reg3b) seven days after tamoxifen administration. Yellow arrowheads indicate Lyz-positive cells on the villi; the white arrowheads in the lower left image show that the crypt bases in Tcf4 + epithelium are not positive for Reg3b, whereas they produce Reg3b in Tcf4-deficient epithelium (white arrowheads in the lower right figure). Scale bar: 50 μm. C) Kaplan-Meier survival curves for Tcf7l2 knockout mice treated with either antibiotics or water after tamoxifen induction. Statistical significance (p = 0.034) shows that antibiotic treatment increases survival. D) Analysis of the composition of the microbiome in the distal small intestine of Tcf7l2 wt/wt and Tcf7l2 flox/flox mice. The pie chart shows the bacterial distribution in Tcf4-proficient mice (a solid black point on the outer perimeter of the chart) and Tcf4-deficient mice (an empty black ring). The principal component analysis (PCA) below the diagram illustrates the variance between bacterial populations, indicating shifts in the microbiota associated with the Tcf7l2 status. Tcf7l2, transcription factor 7 like 2.

    Journal: bioRxiv

    Article Title: The fate of secretory cells during intestinal homeostasis, regeneration, and tumor formation is regulated by Tcf4

    doi: 10.1101/2024.07.11.603019

    Figure Lengend Snippet: A) Fluorescence micrographs of T-cell factor 4 (Tcf4, green) and proliferating cell nuclear antigen (PCNA, red) protein localization in the small intestine of wild-type Tcf7l2 wt/wt /VillinCreER T2 ( Tcf7l2 wt/wt ) and knockout Tcf7l2 flox/flox /VillinCreER T2 ( Tcf7l2 flox/flox ) mice, with antibiotics (ATB) and without antibiotic treatment, over time after tamoxifen administration. The images show disruptions in the epithelial architecture of Tcf4-deficient mice that are partially restored by antibiotic treatment. The insets show increased magnification of the framed areas. Scale bar: 50 μm. B) Histological sections show the mislocalization and expression changes of the Paneth cell markers lysozyme (Lyz) and regenerating islet-derived 3 beta (Reg3b) seven days after tamoxifen administration. Yellow arrowheads indicate Lyz-positive cells on the villi; the white arrowheads in the lower left image show that the crypt bases in Tcf4 + epithelium are not positive for Reg3b, whereas they produce Reg3b in Tcf4-deficient epithelium (white arrowheads in the lower right figure). Scale bar: 50 μm. C) Kaplan-Meier survival curves for Tcf7l2 knockout mice treated with either antibiotics or water after tamoxifen induction. Statistical significance (p = 0.034) shows that antibiotic treatment increases survival. D) Analysis of the composition of the microbiome in the distal small intestine of Tcf7l2 wt/wt and Tcf7l2 flox/flox mice. The pie chart shows the bacterial distribution in Tcf4-proficient mice (a solid black point on the outer perimeter of the chart) and Tcf4-deficient mice (an empty black ring). The principal component analysis (PCA) below the diagram illustrates the variance between bacterial populations, indicating shifts in the microbiota associated with the Tcf7l2 status. Tcf7l2, transcription factor 7 like 2.

    Article Snippet: Primary antibodies: anti-Alpi (rabbit polyclonal, PA5-22210, Thermo Fisher Scientific); anti-ChgA (rabbit polyclonal, ab15160, Abcam, Cambridge, UK); anti-cleaved Casp3 (rabbit monoclonal, #9664, Cell Signaling Technology, Danvers, MA, USA); anti-Krt20 (mouse monoclonal, M7019, Agilent Dako, Santa Clara, CA, USA); anti-Lysozyme (rabbit polyclonal, A0099, Agilent Dako); anti-Muc2 (rabbit polyclonal, sc-15334, Santa Cruz Biotechnology, Dallas, TX, USA); anti-Olfm4 (rabbit monoclonal, #39141, Cell Signaling Technology); anti-PCNA (rabbit polyclonal, ab18197, Abcam); anti-PCNA (mouse monoclonal, ab29, Abcam); anti-Pdgfra (goat polyclonal, AF1062, R&D Systems, Minneapolis, MN, USA); anti-Reg3b (sheep polyclonal, AF5110, R&D Systems); anti-RFP (rabbit polyclonal, 600-401-379, Rockland, Pottstown, PA, USA); anti-RFP (mouse monoclonal, MA5-15257, Thermo Fisher Scientific); anti-Tacstd2 (rabbit monoclonal, ab214488, Abcam); anti-Tcf4 (rabbit monoclonal, #2569, Cell Signaling Technology); anti-Tcf4 (rabbit monoclonal, MA5-35295, Thermo Fisher Scientific).

    Techniques: Fluorescence, Knock-Out, Expressing, Derivative Assay